BAIT
ATG5
2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337, Paddy
autophagy related 5
GO Process (23)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- C-terminal protein lipidation [IBA]
- autophagic vacuole assembly [IMP]
- autophagy [IMP]
- blood vessel remodeling [IMP]
- cellular response to nitrogen starvation [IBA]
- heart contraction [IMP]
- mitochondrion degradation [IBA]
- negative regulation of apoptotic process [IMP]
- negative regulation of histone H4-K16 acetylation [IMP]
- negative regulation of protein ubiquitination [IMP]
- negative stranded viral RNA replication [IMP]
- nucleophagy [IBA]
- otolith development [IMP]
- positive regulation of mucus secretion [IMP]
- post-translational protein modification [IDA]
- regulation of cilium assembly [IMP]
- regulation of cytokine secretion involved in immune response [IMP]
- regulation of reactive oxygen species metabolic process [IMP]
- regulation of release of sequestered calcium ion into cytosol [IMP]
- response to drug [IMP]
- response to fungus [IMP]
- vasodilation [IMP]
- ventricular cardiac muscle cell development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
TNFRSF1A
CD120a, FPF, TNF-R, TNF-R-I, TNF-R1, TNF-R55, TNF-alphaR1, TNFAR, TNFR60, TNFRI, TNFRp55, TNFalpha-R1, Tnfr-2, Tnfr1, p55, p55-R
tumor necrosis factor receptor superfamily, member 1a
GO Process (23)
GO Function (5)
GO Component (13)
Gene Ontology Biological Process
- cell surface receptor signaling pathway [IMP]
- cytokine-mediated signaling pathway [IMP]
- defense response [IMP]
- defense response to bacterium [IMP]
- inflammatory response [IMP]
- intrinsic apoptotic signaling pathway in response to DNA damage [IMP]
- negative regulation of apoptotic process [ISO]
- negative regulation of gene expression [ISO]
- negative regulation of inflammatory response [ISO]
- negative regulation of interleukin-6 production [ISO]
- positive regulation of angiogenesis [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of inflammatory response [IMP]
- positive regulation of neuron apoptotic process [ISO]
- positive regulation of protein import into nucleus, translocation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IMP, ISO]
- positive regulation of tumor necrosis factor production [ISO]
- positive regulation of tyrosine phosphorylation of Stat1 protein [ISO]
- programmed cell death [IPI]
- prostaglandin metabolic process [TAS]
- protein heterooligomerization [ISO]
- regulation of apoptotic process [ISO]
- tumor necrosis factor-mediated signaling pathway [IMP, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Autophagy genes in myeloid cells counteract IFN?-induced TNF-mediated cell death and fatal TNF-induced shock.
Host inflammatory responses must be tightly regulated to ensure effective immunity while limiting tissue injury. IFN gamma (IFN?) primes macrophages to mount robust inflammatory responses. However, IFN? also induces cell death, and the pathways that regulate IFN?-induced cell death are incompletely understood. Using genome-wide CRISPR/Cas9 screening, we identified autophagy genes as central mediators of myeloid cell survival during the IFN? ... [more]
Proc Natl Acad Sci U S A Dec. 13, 2018; 116(33);16497-16506 [Pubmed: 31346084]
Throughput
- High Throughput
Additional Notes
- CRISPR GI screen: Increased ligand resistance
- Cell Line: BV-2 (BTO:0003350) Atg5 knockout (Atg5KO) cells
- Experimental Setup: Ligand Exposure (Interferon gamma)
- Library: Brie (mouse)
- Significance Threshold: Positive STARS score FDR < 0.1
Curated By
- BioGRID