BAIT
ATG5
2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337, Paddy
autophagy related 5
GO Process (23)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- C-terminal protein lipidation [IBA]
- autophagic vacuole assembly [IMP]
- autophagy [IMP]
- blood vessel remodeling [IMP]
- cellular response to nitrogen starvation [IBA]
- heart contraction [IMP]
- mitochondrion degradation [IBA]
- negative regulation of apoptotic process [IMP]
- negative regulation of histone H4-K16 acetylation [IMP]
- negative regulation of protein ubiquitination [IMP]
- negative stranded viral RNA replication [IMP]
- nucleophagy [IBA]
- otolith development [IMP]
- positive regulation of mucus secretion [IMP]
- post-translational protein modification [IDA]
- regulation of cilium assembly [IMP]
- regulation of cytokine secretion involved in immune response [IMP]
- regulation of reactive oxygen species metabolic process [IMP]
- regulation of release of sequestered calcium ion into cytosol [IMP]
- response to drug [IMP]
- response to fungus [IMP]
- vasodilation [IMP]
- ventricular cardiac muscle cell development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
IRG1
AI323667, CAD
immunoresponsive gene 1
GO Process (20)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
- cellular response to interferon-beta [IDA]
- cellular response to interferon-gamma [IDA]
- cellular response to interleukin-1 [IDA]
- cellular response to lipopolysaccharide [IDA, ISO]
- cellular response to molecule of bacterial origin [IDA]
- cellular response to progesterone stimulus [IDA]
- cellular response to tumor necrosis factor [IDA]
- defense response [IDA, ISO]
- embryo implantation [IMP]
- metabolic process [IDA, ISO]
- negative regulation of NF-kappaB transcription factor activity [IMP]
- negative regulation of inflammatory response [IDA]
- negative regulation of innate immune response [IDA]
- negative regulation of toll-like receptor 2 signaling pathway [IMP]
- negative regulation of toll-like receptor 4 signaling pathway [IDA]
- negative regulation of type I interferon production [IMP]
- positive regulation of antimicrobial humoral response [IDA, ISO]
- positive regulation of reactive oxygen species metabolic process [IDA]
- response to lipopolysaccharide [IDA]
- tolerance induction to lipopolysaccharide [IDA, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Autophagy genes in myeloid cells counteract IFN?-induced TNF-mediated cell death and fatal TNF-induced shock.
Host inflammatory responses must be tightly regulated to ensure effective immunity while limiting tissue injury. IFN gamma (IFN?) primes macrophages to mount robust inflammatory responses. However, IFN? also induces cell death, and the pathways that regulate IFN?-induced cell death are incompletely understood. Using genome-wide CRISPR/Cas9 screening, we identified autophagy genes as central mediators of myeloid cell survival during the IFN? ... [more]
Proc Natl Acad Sci U S A Dec. 13, 2018; 116(33);16497-16506 [Pubmed: 31346084]
Throughput
- High Throughput
Additional Notes
- CRISPR GI screen: Increased ligand resistance
- Cell Line: BV-2 (BTO:0003350) Atg5 knockout (Atg5KO) cells
- Experimental Setup: Ligand Exposure (Interferon gamma)
- Library: Brie (mouse)
- Significance Threshold: Positive STARS score FDR < 0.1
Curated By
- BioGRID