An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Quantitative proteomics links the LRRC59 interactome to mRNA translation on the ER membrane.

Hannigan MM, Hoffman AM, Thompson W, Zheng T, Nicchitta CV

Protein synthesis on the endoplasmic reticulum (ER) requires the dynamic coordination of numerous cellular components. Together, resident ER membrane proteins, cytoplasmic translation factors, and both integral membrane and cytosolic RNA-binding proteins operate in concert with membrane-associated ribosomes to facilitate ER-localized translation. Little is known, however, regarding the spatial organization of ER-localized translation. This question is of growing significance as it ... [more]

Mol Cell Proteomics Aug. 11, 2020; (); [Pubmed: 32788342]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RPN1 CLGN	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Wan C (2015)	High	0.5616	BioGRID	1260074
RPN1 CLGN	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Go CD (2021)	High	275	BioGRID	3004794

Curated By

BioGRID

Interaction Summary

RPN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

dolichyl-diphosphooligosaccharide-protein glycotransferase activity [TAS]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

CLGN