An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

14-3-3zeta interacts with the alpha-chain of human interleukin 9 receptor.

Sliva D, Gu M, Zhu YX, Chen J, Tsai S, Du X, Yang YC

Interleukin 9 (IL-9) exerts its pleiotropic effects through the IL-9 receptor (IL-9R) complex, which consists of the IL-9R alpha-chain, which determines the cytokine specificity, and the IL-2 receptor gamma-chain. In the present study we used a modified yeast two-hybrid system to isolate cDNA species encoding proteins that interacted with the intracellular domain of the human IL-9R alpha-chain (hIL-9Ralpha). We have ... [more]

Biochem. J. Feb. 01, 2000; 345(0);741-7 [Pubmed: 10642536]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
YWHAZ IL9R	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Sliva D (2000)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

YWHAZ

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase binding [IPI]transcription factor binding [IPI]

Gene Ontology Cellular Component

IL9R