BAIT

LRRC59

p34, PRO1855
leucine rich repeat containing 59
GO Process (0)
GO Function (1)
GO Component (3)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

EIF3A

EIF3, EIF3S10, P167, TIF32, eIF3-p170, eIF3-theta, p180, p185
eukaryotic translation initiation factor 3, subunit A
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Quantitative proteomics links the LRRC59 interactome to mRNA translation on the ER membrane.

Hannigan MM, Hoffman AM, Thompson W, Zheng T, Nicchitta CV

Protein synthesis on the endoplasmic reticulum (ER) requires the dynamic coordination of numerous cellular components. Together, resident ER membrane proteins, cytoplasmic translation factors, and both integral membrane and cytosolic RNA-binding proteins operate in concert with membrane-associated ribosomes to facilitate ER-localized translation. Little is known, however, regarding the spatial organization of ER-localized translation. This question is of growing significance as it ... [more]

Mol Cell Proteomics Aug. 11, 2020; (); [Pubmed: 32788342]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EIF3A LRRC59
Cross-Linking-MS (XL-MS)
Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

High-BioGRID
3678671

Curated By

  • BioGRID