EIF3E
Gene Ontology Biological Process
Gene Ontology Molecular Function
IFIT1
Gene Ontology Biological Process
- cellular response to exogenous dsRNA [IDA]
- cellular response to type I interferon [IDA]
- cytokine-mediated signaling pathway [TAS]
- intracellular transport of viral protein in host cell [IDA]
- negative regulation of defense response to virus by host [IDA]
- negative regulation of helicase activity [IDA]
- negative regulation of protein binding [IDA]
- negative regulation of viral genome replication [IMP]
- positive regulation of viral genome replication [IDA]
- response to virus [IDA, IMP]
- type I interferon signaling pathway [TAS]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Characterization of the interaction between the interferon-induced protein P56 and the Int6 protein encoded by a locus of insertion of the mouse mammary tumor virus.
For determining cellular functions of the interferon-inducible human cytoplasmic protein P56, we undertook a Saccharomyces cerevisiae two-hybrid screen that identified Int6 as a P56-interacting protein. That the interaction also occurs in human cells was confirmed by coimmunoprecipitation and the observed cytoplasmic displacement of nuclear Int6 upon coexpression of P56. Because Int6 has been claimed to be both a cytoplasmic and ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID