An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Quantitative proteomics links the LRRC59 interactome to mRNA translation on the ER membrane.

Hannigan MM, Hoffman AM, Thompson W, Zheng T, Nicchitta CV

Protein synthesis on the endoplasmic reticulum (ER) requires the dynamic coordination of numerous cellular components. Together, resident ER membrane proteins, cytoplasmic translation factors, and both integral membrane and cytosolic RNA-binding proteins operate in concert with membrane-associated ribosomes to facilitate ER-localized translation. Little is known, however, regarding the spatial organization of ER-localized translation. This question is of growing significance as it ... [more]

Mol Cell Proteomics Aug. 11, 2020; (); [Pubmed: 32788342]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LRRC59 ZC3HAV1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Go CD (2021)	High	10.91	BioGRID	2995335
LRRC59 ZC3HAV1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Hannigan MM (2020)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

LRRC59

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]

Gene Ontology Cellular Component

ZC3HAV1

Gene Ontology Biological Process