An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The N terminus of amphiphysin II mediates dimerization and plasma membrane targeting.

Ramjaun AR, Philie J, de Heuvel E, McPherson PS

Amphiphysin I and II are nerve terminal-enriched proteins containing SH3 domains that interact with dynamin and synaptojanin. The amphiphysins may function in synaptic vesicle endocytosis by targeting synaptojanin and dynamin to emerging endocytic buds through SH3 domain-independent interactions with clathrin and AP2. We have recently identified and cloned several amphiphysin II splice variants that differentially incorporate clathrin-binding domains. To determine ... [more]

J. Biol. Chem. Jul. 09, 1999; 274(28);19785-91 [Pubmed: 10391921]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

BIN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase binding [IPI]identical protein binding [IPI]protein binding [IPI]tau protein binding [IPI]

Gene Ontology Cellular Component