BAIT

HUWE1

ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MULE, URE-B1, UREB1, RP3-339A18.4
HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
Fanconi Anemia Project
Human Papilloma Virus (HPV) Project
UPS Project
Homo sapiens
PREY

ZCCHC2

C18orf49
zinc finger, CCHC domain containing 2
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The zinc-finger protein ZCCHC2 suppresses retinoblastoma tumorigenesis by inhibiting HectH9-mediated K63-linked polyubiquitination and activation of c-Myc.

Dai H, Yan M, Li Y

Retinoblastoma (RB) is the most common intraocular malignancy. The tumor propagation of RB is maintained by several core transcriptional regulators, including c-Myc. Strictly regulated posttranslational modifications control the c-Myc protein. However, the posttranslational regulatory mechanisms for c-Myc in retinoblastoma remain largely unclear. Here, we identified the zinc-finger protein ZCCHC2 as a critical negative regulator of c-Myc-associated tumorigenesis. Knockout of ZCCHC2 ... [more]

Biochem Biophys Res Commun Dec. 08, 2019; 521(2);533-538 [Pubmed: 31677785]

Throughput

  • Low Throughput

Additional Notes

  • Source of HectH9/Huwe1 not clear

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ZCCHC2 HUWE1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
2877726

Curated By

  • BioGRID