An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Cellular redox sensor HSCARG negatively regulates the translesion synthesis pathway and exacerbates mammary tumorigenesis.

Zang W, Yang C, Li T, Liao L, Zheng X

The translesion synthesis (TLS) pathway is a double-edged sword in terms of genome integrity. Deficiency in TLS leads to generation of DNA double strand break (DSB) during replication stress, while excessive activation of the TLS pathway increases the risk of point mutation. Here we demonstrate that HSCARG, a cellular redox sensor, directly interacts with the key protein PCNA in the ... [more]

Proc Natl Acad Sci U S A Dec. 17, 2018; 116(51);25624-25633 [Pubmed: 31796584]

Throughput

Low Throughput

Additional Notes

Interaction is dependent on USP1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
WDR48 NMRAL1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Zang W (2019)	Low	-	BioGRID	2878066

Curated By

BioGRID

Interaction Summary

WDR48

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

NMRAL1