Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

ER-localized Hrd1 ubiquitinates and inactivates Usp15 to promote TLR4-induced inflammation during bacterial infection.

Lu Y, Qiu Y, Chen P, Chang H, Guo L, Zhang F, Ma L, Zhang C, Zheng X, Xiao J, Zhong R, Han L, Xu X, Zhang Y, Li D, Zhong G, Boyton R, Huang Y, He Y, Hu R, Wei B, Wang H

The special organelle-located MAVS, STING and TLR3 are important for clearing viral infections. Although TLR4 triggers NF-?B activation to produce pro-inflammatory cytokines for bacterial clearance, effectors with special organelle localization have not been identified. Here, we screened more than 280 E3 ubiquitin ligases and discovered that the endoplasmic reticulum-located Hrd1 regulates TLR4-induced inflammation during bacterial infection. Hrd1 interacts directly with ... [more]

Nat Microbiol Dec. 01, 2018; 4(12);2331-2346 [Pubmed: 31477895]

Throughput

  • Low Throughput

Additional Notes

  • Interaction dependent on Hrd1/SYVN1

Curated By

  • BioGRID