An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

USP14 is a deubiquitinase for Ku70 and critical determinant of non-homologous end joining repair in autophagy and PTEN-deficient cells.

Sharma A, Alswillah T, Kapoor I, Debjani P, Willard B, Summers MK, Gong Z, Almasan A

Ionizing radiation (IR)-induced DNA double-strand breaks (DSBs) are predominantly repaired by non-homologous end joining (NHEJ). IR-induced DNA damage activates autophagy, an intracellular degradation process that delivers cytoplasmic components to the lysosome. We identified the deubiquitinase USP14 as a novel autophagy substrate and a regulator of IR-induced DNA damage response (DDR) signaling. Inhibition of autophagy increased levels and DSB recruitment of ... [more]

Nucleic Acids Res Dec. 24, 2019; 48(2);736-747 [Pubmed: 31740976]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
USP14 USP14	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Liu B (2018)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

USP14

Gene Ontology Biological Process

Gene Ontology Molecular Function

cysteine-type endopeptidase activity [TAS]endopeptidase inhibitor activity [IMP]proteasome binding [IDA]protein binding [IPI]tRNA guanylyltransferase activity [TAS]ubiquitin thiolesterase activity [IDA]ubiquitin-specific protease activity [IDA]

Gene Ontology Cellular Component