BAIT
KRAS
AI929937, K-ras, Ki-ras, Kras-2, Kras2, p21B, ras, RP24-359O2.1
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
GO Process (18)
GO Function (6)
GO Component (7)
Gene Ontology Biological Process
- GTP catabolic process [IGI]
- Ras protein signal transduction [IGI, TAS]
- actin cytoskeleton organization [IGI]
- cytokine-mediated signaling pathway [ISO]
- epithelial tube branching involved in lung morphogenesis [IDA, IGI]
- negative regulation of cell differentiation [IDA]
- negative regulation of neuron apoptotic process [IGI]
- positive regulation of MAP kinase activity [ISO]
- positive regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of Rac protein signal transduction [IGI]
- positive regulation of cell proliferation [IGI, ISO]
- positive regulation of gene expression [ISO]
- positive regulation of nitric-oxide synthase activity [ISO]
- positive regulation of protein phosphorylation [ISO]
- regulation of long-term neuronal synaptic plasticity [IGI]
- regulation of synaptic transmission, GABAergic [IGI]
- striated muscle cell differentiation [IGI]
- visual learning [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
ATM
AI256621, C030026E19Rik
ataxia telangiectasia mutated
GO Process (35)
GO Function (9)
GO Component (5)
Gene Ontology Biological Process
- DNA damage checkpoint [IMP]
- DNA damage induced protein phosphorylation [ISO]
- DNA repair [IDA, IMP]
- V(D)J recombination [IGI]
- brain development [IGI]
- cell cycle arrest [ISO]
- cellular response to DNA damage stimulus [ISO, TAS]
- cellular response to gamma radiation [ISO]
- double-strand break repair via homologous recombination [IBA]
- female gamete generation [IMP]
- heart development [IGI]
- histone mRNA catabolic process [ISO]
- histone phosphorylation [ISO]
- histone-serine phosphorylation [ISO]
- intrinsic apoptotic signaling pathway in response to DNA damage [IGI, IMP]
- lipoprotein catabolic process [IGI]
- mitotic spindle assembly checkpoint [ISO]
- negative regulation of B cell proliferation [ISO]
- neuron apoptotic process [IGI]
- oocyte development [IMP]
- peptidyl-serine phosphorylation [ISO]
- phosphatidylinositol phosphorylation [ISO]
- phosphatidylinositol-3-phosphate biosynthetic process [ISO]
- positive regulation of DNA damage response, signal transduction by p53 class mediator [ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of neuron apoptotic process [IGI]
- pre-B cell allelic exclusion [IMP]
- protein autophosphorylation [ISO]
- protein phosphorylation [IDA, ISO, TAS]
- regulation of cell cycle [ISO]
- replicative senescence [ISO]
- response to hypoxia [ISO]
- response to ionizing radiation [IGI, ISO]
- signal transduction involved in mitotic G2 DNA damage checkpoint [ISO]
- somitogenesis [IGI]
Gene Ontology Molecular Function- 1-phosphatidylinositol-3-kinase activity [ISO]
- DNA-dependent protein kinase activity [ISO]
- histone serine kinase activity [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein dimerization activity [IBA, ISO]
- protein kinase activity [IDA, TAS]
- protein serine/threonine kinase activity [ISO]
- 1-phosphatidylinositol-3-kinase activity [ISO]
- DNA-dependent protein kinase activity [ISO]
- histone serine kinase activity [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein dimerization activity [IBA, ISO]
- protein kinase activity [IDA, TAS]
- protein serine/threonine kinase activity [ISO]
Mus musculus
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Genome-wide CRISPR Screen to Identify Genes that Suppress Transformation in the Presence of Endogenous KrasG12D.
Cooperating gene mutations are typically required to transform normal cells enabling growth in soft agar or in immunodeficient mice. For example, mutations in Kras and transformation-related protein 53 (Trp53) are known to transform a variety of mesenchymal and epithelial cells in vitro and in vivo. Identifying other genes that can cooperate with oncogenic Kras and substitute for Trp53 mutation has ... [more]
Sci Rep Dec. 20, 2018; 9(1);17220 [Pubmed: 31748650]
Throughput
- High Throughput
Additional Notes
- CRISPR GI screen
- Cell Line: MEF
- Experimental Setup: non-immortal cell line grown on soft agar or in mouse models to look for co-mutations that lead to cell transformation (positive selection)
- GIST: A-phenotypic positive genetic interaction
- Library: Selected targets from Brie and mGECKO
- Significance Threshold: NA
Curated By
- BioGRID