BAIT
KRAS
AI929937, K-ras, Ki-ras, Kras-2, Kras2, p21B, ras, RP24-359O2.1
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
GO Process (18)
GO Function (6)
GO Component (7)
Gene Ontology Biological Process
- GTP catabolic process [IGI]
- Ras protein signal transduction [IGI, TAS]
- actin cytoskeleton organization [IGI]
- cytokine-mediated signaling pathway [ISO]
- epithelial tube branching involved in lung morphogenesis [IDA, IGI]
- negative regulation of cell differentiation [IDA]
- negative regulation of neuron apoptotic process [IGI]
- positive regulation of MAP kinase activity [ISO]
- positive regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of Rac protein signal transduction [IGI]
- positive regulation of cell proliferation [IGI, ISO]
- positive regulation of gene expression [ISO]
- positive regulation of nitric-oxide synthase activity [ISO]
- positive regulation of protein phosphorylation [ISO]
- regulation of long-term neuronal synaptic plasticity [IGI]
- regulation of synaptic transmission, GABAergic [IGI]
- striated muscle cell differentiation [IGI]
- visual learning [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
SLMAP
D330001L02Rik, Slap, mKIAA1601
sarcolemma associated protein
GO Process (0)
GO Function (1)
GO Component (1)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Genome-wide CRISPR Screen to Identify Genes that Suppress Transformation in the Presence of Endogenous KrasG12D.
Cooperating gene mutations are typically required to transform normal cells enabling growth in soft agar or in immunodeficient mice. For example, mutations in Kras and transformation-related protein 53 (Trp53) are known to transform a variety of mesenchymal and epithelial cells in vitro and in vivo. Identifying other genes that can cooperate with oncogenic Kras and substitute for Trp53 mutation has ... [more]
Sci Rep Dec. 20, 2018; 9(1);17220 [Pubmed: 31748650]
Throughput
- High Throughput
Additional Notes
- CRISPR GI screen
- Cell Line: MEF
- Experimental Setup: non-immortal cell line grown on soft agar or in mouse models to look for co-mutations that lead to cell transformation (positive selection)
- GIST: A-phenotypic positive genetic interaction
- Library: Selected targets from Brie and mGECKO
- Significance Threshold: NA
Curated By
- BioGRID