BAIT
PRKAR2A
PKR2, PRKAR2
protein kinase, cAMP-dependent, regulatory, type II, alpha
GO Process (16)
GO Function (5)
GO Component (8)
Gene Ontology Biological Process
- activation of phospholipase C activity [TAS]
- activation of protein kinase A activity [TAS]
- blood coagulation [TAS]
- cellular response to glucagon stimulus [TAS]
- energy reserve metabolic process [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- intracellular signal transduction [TAS]
- negative regulation of cAMP-dependent protein kinase activity [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- regulation of insulin secretion [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
- water transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PRKAR2A
PKR2, PRKAR2
protein kinase, cAMP-dependent, regulatory, type II, alpha
GO Process (16)
GO Function (5)
GO Component (8)
Gene Ontology Biological Process
- activation of phospholipase C activity [TAS]
- activation of protein kinase A activity [TAS]
- blood coagulation [TAS]
- cellular response to glucagon stimulus [TAS]
- energy reserve metabolic process [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- intracellular signal transduction [TAS]
- negative regulation of cAMP-dependent protein kinase activity [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- regulation of insulin secretion [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
- water transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
High-resolution protein-protein interaction mapping using all-versus-all sequencing (AVA-Seq).
Two-hybrid systems can be used for investigating protein-protein interactions and may provide important information about gene products with unknown function. Despite their success in mapping protein interactions, two-hybrid systems have remained mostly untouched by improvements in next-generation DNA sequencing. The two-hybrid systems rely on one-versus-all methods in which each bait is sequentially screened against an entire library. Here, we developed ... [more]
J Biol Chem Dec. 26, 2018; 294(30);11549-11558 [Pubmed: 31182485]
Throughput
- High Throughput
Curated By
- BioGRID