An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Interactions between two regulatory proteins of microtubule dynamics, HDAC6, TPPP/p25, and the hub protein, DYNLL/LC8.

Olah J, Szunyogh S, Szenasi T, Szaniszlo T, Szabo A, Lehotzky A, Berki T, Nyitray L, Ovadi J

Degradation of unwanted proteins is important in protein quality control cooperating with the dynein/dynactin-mediated trafficking along the acetylated microtubule (MT) network. Proteins associated directly/indirectly with tubulin/MTs play crucial roles in both physiological and pathological processes. Our studies focus on the interrelationship of the tubulin deacetylase HDAC6, the MT-associated TPPP/p25 with its deacetylase inhibitory potency and the hub dynein light chain ... [more]

Biochim Biophys Acta Mol Cell Res Dec. 01, 2018; 1866(12);118556 [Pubmed: 31505170]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TPPP

Gene Ontology Biological Process

Gene Ontology Molecular Function

microtubule binding [ISS]protein binding [IPI]tubulin binding [IDA, ISS]

Gene Ontology Cellular Component