Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

EHSH1/intersectin, a protein that contains EH and SH3 domains and binds to dynamin and SNAP-25. A protein connection between exocytosis and endocytosis?

Okamoto M, Schoch S, Suedhof TC

In yeast two-hybrid screens for proteins that bind to SNAP-25 and may be involved in exocytosis, we isolated a protein called EHSH1 (for EH domain/SH3 domain-containing protein). Cloning of full-length cDNAs revealed that EHSH1 is composed of an N-terminal region with two EH domains, a central region that is enriched in lysine, leucine, glutamate, arginine, and glutamine (KLERQ domain), and ... [more]

J. Biol. Chem. Jun. 25, 1999; 274(26);18446-54 [Pubmed: 10373452]

Throughput

  • Low Throughput

Curated By

  • BioGRID