An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Elucidation of the AGR2 Interactome in Esophageal Adenocarcinoma Cells Identifies a Redox-Sensitive Chaperone Hub for the Quality Control of MUC-5AC.

Worfolk JC, Bell S, Simpson LD, Carne NA, Francis SL, Engelbertsen V, Brown AP, Walker J, Viswanath YK, Benham AM

Aims: AGR2 is a tissue-restricted member of the protein disulfide isomerase family that has attracted interest ... [more]

Antioxid Redox Signal Dec. 20, 2018; 31(15);1117-1132 [Pubmed: 31436131]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

ALDH3A1

Gene Ontology Biological Process

Gene Ontology Molecular Function

3-chloroallyl aldehyde dehydrogenase activity [IBA]alcohol dehydrogenase (NADP+) activity [IDA]aldehyde dehydrogenase (NAD) activity [IDA]

Gene Ontology Cellular Component

ANXA2

Gene Ontology Biological Process

Gene Ontology Molecular Function

S100 protein binding [IPI]calcium-dependent phospholipid binding [IDA]calcium-dependent protein binding [IPI]phosphatidylinositol-4,5-bisphosphate binding [IMP]phospholipase A2 inhibitor activity [IDA]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Elucidation of the AGR2 Interactome in Esophageal Adenocarcinoma Cells Identifies a Redox-Sensitive Chaperone Hub for the Quality Control of MUC-5AC.

Throughput

Curated By

3-chloroallyl aldehyde dehydrogenase activity [IBA]
alcohol dehydrogenase (NADP+) activity [IDA]
aldehyde dehydrogenase (NAD) activity [IDA]

S100 protein binding [IPI]
calcium-dependent phospholipid binding [IDA]
calcium-dependent protein binding [IPI]
phosphatidylinositol-4,5-bisphosphate binding [IMP]
phospholipase A2 inhibitor activity [IDA]
poly(A) RNA binding [IDA]
protein binding [IPI]