BAIT
HULC
HCCAT1, LINC00078, NCRNA00078
hepatocellular carcinoma up-regulated long non-coding RNA
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY
TAPBP
NGS17, TAPA, TPN, TPSN, DADB-159G18.7
TAP binding protein (tapasin)
GO Process (9)
GO Function (7)
GO Component (7)
Gene Ontology Biological Process
- amide transport [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- immune response [TAS]
- peptide antigen stabilization [ISS]
- peptide transport [TAS]
- protein complex assembly [TAS]
- retrograde vesicle-mediated transport, Golgi to ER [NAS]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.
Interacting with proteins is a crucial way for long noncoding RNAs (lncRNAs) to exert their biological responses. Here we report a high throughput strategy to characterize lncRNA interacting proteins in vivo by combining tobramycin affinity purification and mass spectrometric analysis (TOBAP-MS). Using this method, we identify 140 candidate binding proteins for lncRNA highly upregulated in liver cancer (HULC). Intriguingly, HULC ... [more]
Nat Commun Dec. 22, 2019; 11(1);3162 [Pubmed: 32572027]
Throughput
- High Throughput
Additional Notes
- Tobramycin affinity purification of lncRNA-HULC
Curated By
- BioGRID