BAIT

EHD3

MRP5, YDR036C
3-hydroxyisobutyryl-CoA hydrolase; member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

MRP51

mitochondrial 37S ribosomal protein MRP51, L000003325, YPL118W
Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Molecular Connectivity of Mitochondrial Gene Expression and OXPHOS Biogenesis.

Singh AP, Salvatori R, Aftab W, Aufschnaiter A, Carlstroem A, Forne I, Imhof A, Ott M

Mitochondria contain their own gene expression systems, including membrane-bound ribosomes dedicated to synthesizing a few hydrophobic subunits of the oxidative phosphorylation (OXPHOS) complexes. We used a proximity-dependent biotinylation technique, BioID, coupled with mass spectrometry to delineate in baker's yeast a comprehensive network of factors involved in biogenesis of mitochondrial encoded proteins. This mitochondrial gene expression network (MiGENet) encompasses proteins involved ... [more]

Mol Cell Dec. 17, 2019; 79(6);1051-1065.e10 [Pubmed: 32877643]

Quantitative Score

  • 3.906952557 [log2fold change (FC)]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • Proximal mitochondrial proteins were detected using BioID analyses and proteins with a log2fold change >= 1.5 and a p-value =< 0.05 were considered significant.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EHD3 MRP51
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
EHD3 MRP51
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
EHD3 MRP51
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High10BioGRID
3594136

Curated By

  • BioGRID