BAIT

MRP13

mitochondrial 37S ribosomal protein YmS-A, L000001142, L000001157, YGR084C
Mitochondrial ribosomal protein of the small subunit
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

GEP3

AIM40, FMP38, MTG3, LRC5, YOR205C
Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (Phb1p)
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Molecular Connectivity of Mitochondrial Gene Expression and OXPHOS Biogenesis.

Singh AP, Salvatori R, Aftab W, Aufschnaiter A, Carlstroem A, Forne I, Imhof A, Ott M

Mitochondria contain their own gene expression systems, including membrane-bound ribosomes dedicated to synthesizing a few hydrophobic subunits of the oxidative phosphorylation (OXPHOS) complexes. We used a proximity-dependent biotinylation technique, BioID, coupled with mass spectrometry to delineate in baker's yeast a comprehensive network of factors involved in biogenesis of mitochondrial encoded proteins. This mitochondrial gene expression network (MiGENet) encompasses proteins involved ... [more]

Mol Cell Dec. 17, 2019; 79(6);1051-1065.e10 [Pubmed: 32877643]

Quantitative Score

  • 2.215895622 [log2fold change (FC)]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • Proximal mitochondrial proteins were detected using BioID analyses and proteins with a log2fold change >= 1.5 and a p-value =< 0.05 were considered significant.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MRP13 GEP3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High4BioGRID
3619278
GEP3 MRP13
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1604BioGRID
2185470

Curated By

  • BioGRID