BAIT

OXA1

PET1402, L000001318, YER154W
Mitochondrial inner membrane insertase; mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane; also has a role in insertion of carrier proteins into the inner membrane; acts as a voltage-gated ion channel, activated by substrate peptides; interacts with mitochondrial ribosomes; conserved from bacteria to animals
Saccharomyces cerevisiae (S288c)
PREY

FMP52

YER004W
Protein of unknown function; localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation
GO Process (0)
GO Function (0)
GO Component (3)

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Molecular Connectivity of Mitochondrial Gene Expression and OXPHOS Biogenesis.

Singh AP, Salvatori R, Aftab W, Aufschnaiter A, Carlstroem A, Forne I, Imhof A, Ott M

Mitochondria contain their own gene expression systems, including membrane-bound ribosomes dedicated to synthesizing a few hydrophobic subunits of the oxidative phosphorylation (OXPHOS) complexes. We used a proximity-dependent biotinylation technique, BioID, coupled with mass spectrometry to delineate in baker's yeast a comprehensive network of factors involved in biogenesis of mitochondrial encoded proteins. This mitochondrial gene expression network (MiGENet) encompasses proteins involved ... [more]

Mol Cell Dec. 17, 2019; 79(6);1051-1065.e10 [Pubmed: 32877643]

Quantitative Score

  • 1.588645255 [log2fold change (FC)]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • Proximal mitochondrial proteins were detected using BioID analyses and proteins with a log2fold change >= 1.5 and a p-value =< 0.05 were considered significant.

Curated By

  • BioGRID