BAIT

OXA1

PET1402, L000001318, YER154W
Mitochondrial inner membrane insertase; mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane; also has a role in insertion of carrier proteins into the inner membrane; acts as a voltage-gated ion channel, activated by substrate peptides; interacts with mitochondrial ribosomes; conserved from bacteria to animals
Saccharomyces cerevisiae (S288c)
PREY

PHB2

prohibitin subunit PHB2, L000004019, YGR231C
Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR)
GO Process (4)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Molecular Connectivity of Mitochondrial Gene Expression and OXPHOS Biogenesis.

Singh AP, Salvatori R, Aftab W, Aufschnaiter A, Carlstroem A, Forne I, Imhof A, Ott M

Mitochondria contain their own gene expression systems, including membrane-bound ribosomes dedicated to synthesizing a few hydrophobic subunits of the oxidative phosphorylation (OXPHOS) complexes. We used a proximity-dependent biotinylation technique, BioID, coupled with mass spectrometry to delineate in baker's yeast a comprehensive network of factors involved in biogenesis of mitochondrial encoded proteins. This mitochondrial gene expression network (MiGENet) encompasses proteins involved ... [more]

Mol Cell Dec. 17, 2019; 79(6);1051-1065.e10 [Pubmed: 32877643]

Quantitative Score

  • 1.992926865 [log2fold change (FC)]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • Proximal mitochondrial proteins were detected using BioID analyses and proteins with a log2fold change >= 1.5 and a p-value =< 0.05 were considered significant.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PHB2 OXA1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High2BioGRID
3620925
OXA1 PHB2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2063BioGRID
2110532

Curated By

  • BioGRID