BAIT
MAFB
KRML, MCTO
v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog B
GO Process (2)
GO Function (2)
GO Component (0)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
RPL7
L7, humL7-1
ribosomal protein L7
GO Process (15)
GO Function (7)
GO Component (7)
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- SRP-dependent cotranslational protein targeting to membrane [TAS]
- cellular protein metabolic process [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]
- rRNA processing [IMP]
- ribosomal large subunit biogenesis [IMP]
- translation [NAS, TAS]
- translational elongation [TAS]
- translational initiation [TAS]
- translational termination [TAS]
- viral life cycle [TAS]
- viral process [TAS]
- viral transcription [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The deubiquitinase USP7 stabilizes Maf proteins to promote myeloma cell survival.
The Maf proteins, including c-Maf, MafA, and MafB, are critical transcription factors in myelomagenesis. Previous studies demonstrated that Maf proteins are processed by the ubiquitin-proteasome pathway, but the mechanisms remain elusive. This study applied MS to identify MafB ubiquitination-associated proteins and found that the ubiquitin-specific protease USP7 was present in the MafB interactome. Moreover, USP7 also interacted with c-Maf and ... [more]
J Biol Chem Dec. 14, 2019; 295(7);2084-2096 [Pubmed: 31822558]
Throughput
- High Throughput
Additional Notes
- hit/prey protein identified by mass spectrometry with at least 2 unique peptides identified with a p value of <0.05 in both MafB and MafB + MG132 immunoprecipitations
Curated By
- BioGRID