BAIT

MAFB

KRML, MCTO
v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog B
GO Process (2)
GO Function (2)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens
PREY

PIP4K2A

PI5P4KA, PIP5K2A, PIP5KII-alpha, PIP5KIIA, PIPK, RP11-301N24.1
phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
Autophagy Project
GO Process (4)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The deubiquitinase USP7 stabilizes Maf proteins to promote myeloma cell survival.

He Y, Wang S, Tong J, Jiang S, Yang Y, Zhang Z, Xu Y, Zeng Y, Cao B, Moran MF, Mao X

The Maf proteins, including c-Maf, MafA, and MafB, are critical transcription factors in myelomagenesis. Previous studies demonstrated that Maf proteins are processed by the ubiquitin-proteasome pathway, but the mechanisms remain elusive. This study applied MS to identify MafB ubiquitination-associated proteins and found that the ubiquitin-specific protease USP7 was present in the MafB interactome. Moreover, USP7 also interacted with c-Maf and ... [more]

J Biol Chem Dec. 14, 2019; 295(7);2084-2096 [Pubmed: 31822558]

Throughput

  • High Throughput

Additional Notes

  • hit/prey protein identified by mass spectrometry with at least 2 unique peptides identified with a p value of <0.05 in both MafB and MafB + MG132 immunoprecipitations

Curated By

  • BioGRID