BAIT
MAFB
KRML, MCTO
v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog B
GO Process (2)
GO Function (2)
GO Component (0)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
YWHAG
14-3-3GAMMA, PPP1R170
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma
GO Process (11)
GO Function (5)
GO Component (5)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- apoptotic process [TAS]
- intrinsic apoptotic signaling pathway [TAS]
- membrane organization [TAS]
- mitotic cell cycle [TAS]
- negative regulation of protein kinase activity [NAS]
- negative regulation of protein serine/threonine kinase activity [NAS]
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [TAS]
- regulation of neuron differentiation [IMP]
- regulation of signal transduction [NAS]
- regulation of synaptic plasticity [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The deubiquitinase USP7 stabilizes Maf proteins to promote myeloma cell survival.
The Maf proteins, including c-Maf, MafA, and MafB, are critical transcription factors in myelomagenesis. Previous studies demonstrated that Maf proteins are processed by the ubiquitin-proteasome pathway, but the mechanisms remain elusive. This study applied MS to identify MafB ubiquitination-associated proteins and found that the ubiquitin-specific protease USP7 was present in the MafB interactome. Moreover, USP7 also interacted with c-Maf and ... [more]
J Biol Chem Dec. 14, 2019; 295(7);2084-2096 [Pubmed: 31822558]
Throughput
- High Throughput
Additional Notes
- hit/prey protein identified by mass spectrometry with at least 2 unique peptides identified with a p value of <0.05 in both MafB and MafB + MG132 immunoprecipitations
Curated By
- BioGRID