An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

?-Actin mRNA interactome mapping by proximity biotinylation.

Mukherjee J, Hermesh O, Eliscovich C, Nalpas N, Franz-Wachtel M, Macek B, Jansen RP

The molecular function and fate of mRNAs are controlled by RNA-binding proteins (RBPs). Identification of the interacting proteome of a specific mRNA in vivo remains very challenging, however. Based on the widely used technique of RNA tagging with MS2 aptamers for RNA visualization, we developed a RNA proximity biotinylation (RNA-BioID) technique by tethering biotin ligase (BirA*) via MS2 coat protein ... [more]

Proc Natl Acad Sci U S A Dec. 25, 2018; 116(26);12863-12872 [Pubmed: 31189591]

Throughput

High Throughput|Low Throughput

Additional Notes

Bait is ACTB mRNA; hit identified by western (LTP) or MS (HTP)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
IGF2BP1 ACTB	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Mukherjee J (2019)	Low	-	BioGRID	-
IGF2BP1 ACTB	Protein-RNA Protein-RNA An interaction is detected between and protein and an RNA in vitro.	Mukherjee J (2019)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ACTB

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

IGF2BP1