ADAM12
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
IGFBP3
Gene Ontology Biological Process
- cellular protein metabolic process [TAS]
- negative regulation of cell proliferation [IGI]
- negative regulation of protein phosphorylation [IDA]
- negative regulation of signal transduction [NAS]
- negative regulation of smooth muscle cell migration [IDA]
- negative regulation of smooth muscle cell proliferation [IDA]
- positive regulation of apoptotic process [IMP]
- positive regulation of catalytic activity [IDA]
- positive regulation of myoblast differentiation [IDA]
- protein phosphorylation [IDA]
- regulation of insulin-like growth factor receptor signaling pathway [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Biochemical Activity (Proteolytic Processing)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
ADAM 12-S cleaves IGFBP-3 and IGFBP-5 and is inhibited by TIMP-3.
ADAMs are a family of multidomain proteins having proteolytic and cell adhesion activities. We have previously shown that ADAM 12-S, the secreted soluble form of human ADAM 12, is a catalytically active protease. We now describe the purification of full-length recombinant ADAM 12-S and demonstrate that it cleaves insulin-like growth factor binding protein-3 (IGFBP-3). This result supports a role for ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
IGFBP3 ADAM12 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
IGFBP3 ADAM12 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID