BAIT

TCF7L2

TCF-4, TCF4, RP11-357H24.1
transcription factor 7-like 2 (T-cell specific, HMG-box)
GO Process (28)
GO Function (13)
GO Component (7)
Homo sapiens
PREY

JUP

ARVD12, CTNNG, DP3, DPIII, PDGB, PKGB
junction plakoglobin
GO Process (20)
GO Function (9)
GO Component (17)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The transcriptional factor Tcf-4 contains different binding sites for beta-catenin and plakoglobin.

Miravet S, Piedra J, Miro F, Itarte E, Garcia de Herreros A, Dunach M

beta-Catenin and plakoglobin are two related armadillo proteins necessary for the establishment of adhesion junctions and desmosomes. Moreover, beta-catenin can also act as a transcriptional co-activator through its interaction with the members of Tcf/LEF-1 transcriptional factor family. We show here that Tcf-4 can be phosphorylated in vitro by protein kinase CK2 stoichiometrically in amino acids Ser-58-Ser-59-Ser-60. Phosphorylation of these residues ... [more]

J. Biol. Chem. Jan. 18, 2002; 277(3);1884-91 [Pubmed: 11711551]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TCF7L2 JUP
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2730960

Curated By

  • BioGRID