BAIT

PIP1B

ATHH2, F4I18.6, NAMED PLASMA MEMBRANE INTRINSIC PROTEIN 1;2, PIP1;2, TMP-A, TRANSMEMBRANE PROTEIN A, plasma membrane intrinsic protein 1B, AT2G45960
aquaporin PIP1-2
GO Process (5)
GO Function (1)
GO Component (5)
Arabidopsis thaliana (Columbia)
PREY

PIP2E

PIP2;6, PLASMA MEMBRANE INTRINSIC PROTEIN 2;6, T7F6.18, T7F6_18, plasma membrane intrinsic protein 2E, AT2G39010
plasma membrane intrinsic protein 2E
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Novel Aquaporin Regulatory Mechanisms Revealed by Interactomics.

Bellati J, Champeyroux C, Hem S, Rofidal V, Krouk G, Maurel C, Santoni V

PIP1;2 and PIP2;1 are aquaporins that are highly expressed in roots and bring a major contribution to root water transport and its regulation by hormonal and abiotic factors. Interactions between cellular proteins or with other macromolecules contribute to forming molecular machines. Proteins that molecularly interact with PIP1;2 and PIP2;1 were searched to get new insights into regulatory mechanisms of root ... [more]

Mol Cell Proteomics Dec. 01, 2015; 15(11);3473-3487 [Pubmed: 27609422]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PIP2E PIP1B
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID