LDLRAP1
Gene Ontology Biological Process
- amyloid precursor protein metabolic process [IMP]
- cholesterol homeostasis [IMP]
- cholesterol metabolic process [NAS]
- positive regulation of cholesterol metabolic process [IC]
- positive regulation of receptor-mediated endocytosis [IMP]
- positive regulation of signal transduction [IDA]
- receptor internalization [IMP]
- receptor-mediated endocytosis [IDA]
- receptor-mediated endocytosis of low-density lipoprotein particle involved in cholesterol transport [IMP]
- regulation of establishment of protein localization to plasma membrane [IMP]
- regulation of protein binding [IMP]
- transport [NAS]
Gene Ontology Molecular Function- AP-2 adaptor complex binding [IDA]
- beta-amyloid binding [IPI]
- clathrin adaptor activity [IDA]
- clathrin binding [IDA]
- low-density lipoprotein particle receptor binding [IPI]
- phosphatidylinositol-4,5-bisphosphate binding [IDA]
- phosphotyrosine binding [IDA]
- protein binding [IPI]
- receptor signaling complex scaffold activity [IMP]
- signaling adaptor activity [IDA]
- AP-2 adaptor complex binding [IDA]
- beta-amyloid binding [IPI]
- clathrin adaptor activity [IDA]
- clathrin binding [IDA]
- low-density lipoprotein particle receptor binding [IPI]
- phosphatidylinositol-4,5-bisphosphate binding [IDA]
- phosphotyrosine binding [IDA]
- protein binding [IPI]
- receptor signaling complex scaffold activity [IMP]
- signaling adaptor activity [IDA]
Gene Ontology Cellular Component
CLTC
Gene Ontology Biological Process
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- intracellular protein transport [NAS]
- membrane organization [TAS]
- mitotic nuclear division [IMP]
- negative regulation of hyaluronan biosynthetic process [IDA, IMP]
- negative regulation of protein localization to plasma membrane [IMP]
- osteoblast differentiation [IDA]
- post-Golgi vesicle-mediated transport [TAS]
- receptor internalization [IMP]
- receptor-mediated endocytosis [IMP]
- transferrin transport [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- clathrin coat [NAS]
- clathrin complex [IDA]
- clathrin-coated endocytic vesicle membrane [TAS]
- clathrin-coated vesicle [IDA]
- cytoplasm [IDA]
- cytosol [TAS]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- intracellular membrane-bounded organelle [IDA]
- membrane [IDA]
- plasma membrane [TAS]
- protein complex [IDA]
- spindle [IDA]
- trans-Golgi network membrane [TAS]
- vesicle [IDA]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
ARH is a modular adaptor protein that interacts with the LDL receptor, clathrin, and AP-2.
Mutations in the phosphotyrosine binding domain protein ARH cause autosomal recessive hypercholesterolemia, a disorder caused by defective internalization of low density lipoprotein receptors (LDLR) in the liver. To examine the function of ARH, we used pull-down experiments to test for interactions between ARH, the LDLR, and proteins involved in clathrin-mediated endocytosis. The phosphotyrosine binding domain of ARH interacted with the ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID