LDLRAP1
Gene Ontology Biological Process
- amyloid precursor protein metabolic process [IMP]
- cholesterol homeostasis [IMP]
- cholesterol metabolic process [NAS]
- positive regulation of cholesterol metabolic process [IC]
- positive regulation of receptor-mediated endocytosis [IMP]
- positive regulation of signal transduction [IDA]
- receptor internalization [IMP]
- receptor-mediated endocytosis [IDA]
- receptor-mediated endocytosis of low-density lipoprotein particle involved in cholesterol transport [IMP]
- regulation of establishment of protein localization to plasma membrane [IMP]
- regulation of protein binding [IMP]
- transport [NAS]
Gene Ontology Molecular Function- AP-2 adaptor complex binding [IDA]
- beta-amyloid binding [IPI]
- clathrin adaptor activity [IDA]
- clathrin binding [IDA]
- low-density lipoprotein particle receptor binding [IPI]
- phosphatidylinositol-4,5-bisphosphate binding [IDA]
- phosphotyrosine binding [IDA]
- protein binding [IPI]
- receptor signaling complex scaffold activity [IMP]
- signaling adaptor activity [IDA]
- AP-2 adaptor complex binding [IDA]
- beta-amyloid binding [IPI]
- clathrin adaptor activity [IDA]
- clathrin binding [IDA]
- low-density lipoprotein particle receptor binding [IPI]
- phosphatidylinositol-4,5-bisphosphate binding [IDA]
- phosphotyrosine binding [IDA]
- protein binding [IPI]
- receptor signaling complex scaffold activity [IMP]
- signaling adaptor activity [IDA]
Gene Ontology Cellular Component
LDLR
Gene Ontology Biological Process
- cholesterol homeostasis [IMP]
- cholesterol import [ISS]
- cholesterol transport [IMP]
- endocytosis [TAS]
- intestinal cholesterol absorption [IMP]
- lipid metabolic process [TAS]
- lipoprotein metabolic process [TAS]
- low-density lipoprotein particle clearance [IMP]
- phospholipid transport [ISS]
- phototransduction, visible light [TAS]
- positive regulation of triglyceride biosynthetic process [ISS]
- receptor-mediated endocytosis [TAS]
- regulation of phosphatidylcholine catabolic process [ISS]
- retinoid metabolic process [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- cell surface [IDA]
- clathrin-coated endocytic vesicle membrane [TAS]
- coated pit [IDA]
- early endosome [IDA]
- endosome membrane [TAS]
- external side of plasma membrane [IDA]
- integral component of plasma membrane [TAS]
- late endosome [IDA]
- lysosome [IDA]
- membrane [IDA]
- plasma membrane [TAS]
- receptor complex [IDA]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
ARH is a modular adaptor protein that interacts with the LDL receptor, clathrin, and AP-2.
Mutations in the phosphotyrosine binding domain protein ARH cause autosomal recessive hypercholesterolemia, a disorder caused by defective internalization of low density lipoprotein receptors (LDLR) in the liver. To examine the function of ARH, we used pull-down experiments to test for interactions between ARH, the LDLR, and proteins involved in clathrin-mediated endocytosis. The phosphotyrosine binding domain of ARH interacted with the ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID