BAIT
MAPK1
Erk2
mitogen activated protein kinase 1
GO Process (37)
GO Function (12)
GO Component (19)
Gene Ontology Biological Process
- B cell receptor signaling pathway [ISO]
- ERBB signaling pathway [ISO]
- ERK1 and ERK2 cascade [ISO]
- MAPK cascade [IMP, ISO]
- MAPK import into nucleus [IDA]
- T cell receptor signaling pathway [ISO]
- caveolin-mediated endocytosis [TAS]
- cellular response to DNA damage stimulus [ISO]
- cellular response to granulocyte macrophage colony-stimulating factor stimulus [ISO]
- cellular response to organic substance [IDA]
- cytosine metabolic process [ISO]
- intracellular signal transduction [IDA, TAS]
- labyrinthine layer blood vessel development [ISO]
- lipopolysaccharide-mediated signaling pathway [ISO]
- mammary gland epithelial cell proliferation [ISO]
- negative regulation of cell differentiation [ISO]
- organ morphogenesis [ISO]
- peptidyl-serine phosphorylation [IDA, IMP, ISO]
- peptidyl-threonine phosphorylation [ISO, ISS]
- positive regulation of cell migration [IEP]
- positive regulation of cell proliferation [IEP]
- positive regulation of peptidyl-threonine phosphorylation [ISO]
- positive regulation of transcription, DNA-templated [IEP]
- positive regulation of translation [IMP]
- protein phosphorylation [IDA, ISO, TAS]
- regulation of Golgi inheritance [TAS]
- regulation of cytoskeleton organization [TAS]
- regulation of early endosome to late endosome transport [TAS]
- regulation of protein stability [ISS]
- regulation of stress-activated MAPK cascade [TAS]
- response to epidermal growth factor [ISO, ISS]
- response to estrogen [IDA]
- response to exogenous dsRNA [ISO]
- response to lipopolysaccharide [ISO]
- response to toxic substance [IDA]
- sensory perception of pain [IMP]
- signal transduction [IDA]
Gene Ontology Molecular Function- ATP binding [IDA]
- MAP kinase activity [IDA, IMP, ISO, TAS]
- RNA polymerase II carboxy-terminal domain kinase activity [ISO]
- kinase activity [ISO, TAS]
- mitogen-activated protein kinase kinase kinase binding [IPI]
- phosphatase binding [ISO]
- phosphotyrosine binding [ISO]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein kinase binding [IPI]
- protein serine/threonine kinase activity [IC, IDA, ISO, TAS]
- transcription factor binding [IPI]
- ATP binding [IDA]
- MAP kinase activity [IDA, IMP, ISO, TAS]
- RNA polymerase II carboxy-terminal domain kinase activity [ISO]
- kinase activity [ISO, TAS]
- mitogen-activated protein kinase kinase kinase binding [IPI]
- phosphatase binding [ISO]
- phosphotyrosine binding [ISO]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein kinase binding [IPI]
- protein serine/threonine kinase activity [IC, IDA, ISO, TAS]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
- Golgi apparatus [TAS]
- axon [IDA]
- caveola [TAS]
- cytoplasm [ISO, ISS]
- cytoskeleton [TAS]
- cytosol [IDA, ISO, TAS]
- dendrite cytoplasm [IDA]
- early endosome [TAS]
- extracellular vesicular exosome [ISO]
- focal adhesion [TAS]
- late endosome [TAS]
- microtubule cytoskeleton [ISO]
- mitochondrion [ISO, TAS]
- mitotic spindle [ISS]
- nucleoplasm [IDA, ISO, TAS]
- nucleus [IDA, ISO, TAS]
- perikaryon [IDA]
- protein complex [IDA]
- pseudopodium [ISO]
Rattus norvegicus
PREY
GSK3B
glycogen synthase kinase 3 beta
GO Process (56)
GO Function (17)
GO Component (19)
Gene Ontology Biological Process
- ER overload response [ISO]
- Wnt signaling pathway [ISO]
- axonogenesis [ISO]
- canonical Wnt signaling pathway [ISO]
- canonical Wnt signaling pathway involved in positive regulation of apoptotic process [ISO]
- cell migration [ISO]
- cellular response to interleukin-3 [ISO, ISS]
- cellular response to mechanical stimulus [IDA]
- circadian rhythm [ISO, ISS]
- epithelial to mesenchymal transition [ISO, ISS]
- establishment of cell polarity [IDA]
- establishment or maintenance of cell polarity [IDA]
- extrinsic apoptotic signaling pathway in absence of ligand [ISO, ISS]
- fat cell differentiation [ISO]
- glycogen metabolic process [ISO]
- hippocampus development [ISO]
- hypermethylation of CpG island [ISO]
- intracellular signal transduction [ISO]
- myoblast fusion [ISO]
- myotube differentiation [ISO]
- negative regulation of MAP kinase activity [IMP]
- negative regulation of NFAT protein import into nucleus [ISO, ISS]
- negative regulation of apoptotic process [ISO]
- negative regulation of cardiac muscle hypertrophy [ISO]
- negative regulation of dendrite morphogenesis [IMP]
- negative regulation of neuron maturation [ISO]
- negative regulation of neuron projection development [ISO]
- negative regulation of protein binding [ISO]
- negative regulation of protein complex assembly [ISO]
- organ morphogenesis [ISO]
- peptidyl-serine phosphorylation [ISO, ISS]
- phosphorylation [ISO]
- positive regulation of Rac GTPase activity [ISO]
- positive regulation of apoptotic process [IDA, IMP]
- positive regulation of axon extension [ISO]
- positive regulation of cell-matrix adhesion [ISO]
- positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway [ISO, ISS]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of peptidyl-threonine phosphorylation [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein binding [ISO, ISS]
- positive regulation of protein complex assembly [ISO]
- positive regulation of protein export from nucleus [ISO]
- positive regulation of stem cell differentiation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- protein export from nucleus [ISO]
- protein localization to microtubule [ISO]
- protein phosphorylation [IDA, ISO]
- re-entry into mitotic cell cycle [ISO]
- regulation of gene expression by genetic imprinting [ISO]
- regulation of microtubule-based process [ISO, ISS]
- regulation of neuron projection development [ISO]
- regulation of neuronal synaptic plasticity [IMP]
- response to drug [IEP]
- response to lithium ion [IEP]
- superior temporal gyrus development [ISO]
Gene Ontology Molecular Function- ATP binding [IDA]
- NF-kappaB binding [ISO]
- RNA polymerase II transcription factor binding [ISO]
- beta-catenin binding [ISO]
- integrin binding [IPI]
- ionotropic glutamate receptor binding [IPI]
- kinase activity [ISO, ISS]
- p53 binding [ISO]
- protein binding [IPI]
- protein kinase A catalytic subunit binding [ISO]
- protein kinase activity [ISO]
- protein kinase binding [IPI, ISO]
- protein serine/threonine kinase activity [IDA, ISO, TAS]
- tau protein binding [IDA]
- tau-protein kinase activity [IDA, ISO]
- transcription factor binding [IPI]
- ubiquitin protein ligase binding [ISO]
- ATP binding [IDA]
- NF-kappaB binding [ISO]
- RNA polymerase II transcription factor binding [ISO]
- beta-catenin binding [ISO]
- integrin binding [IPI]
- ionotropic glutamate receptor binding [IPI]
- kinase activity [ISO, ISS]
- p53 binding [ISO]
- protein binding [IPI]
- protein kinase A catalytic subunit binding [ISO]
- protein kinase activity [ISO]
- protein kinase binding [IPI, ISO]
- protein serine/threonine kinase activity [IDA, ISO, TAS]
- tau protein binding [IDA]
- tau-protein kinase activity [IDA, ISO]
- transcription factor binding [IPI]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
- beta-catenin destruction complex [IDA, ISO]
- cell body [ISO]
- centrosome [ISO]
- cytoplasm [IDA, ISO, ISS, TAS]
- cytosol [IDA, ISO, TAS]
- dendritic shaft [ISO]
- dendritic spine [IDA]
- growth cone [ISO]
- membrane [IDA]
- membrane raft [IDA]
- membrane-bounded organelle [ISO]
- neuronal cell body [ISO]
- neuronal postsynaptic density [ISO]
- nucleoplasm [ISO]
- nucleus [IDA, ISO, ISS]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
- protein complex [IDA]
- ribonucleoprotein complex [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Structural Analysis of Hippocampal Kinase Signal Transduction.
Kinases are a major clinical target for human diseases. Identifying the proteins that interact with kinases in vivo will provide information on unreported substrates and will potentially lead to more specific methods for therapeutic kinase regulation. Here, endogenous immunoprecipitations of evolutionally distinct kinases (i.e., Akt, ERK2, and CAMK2) from rodent hippocampi were analyzed by mass spectrometry to generate three highly ... [more]
ACS Chem Neurosci Dec. 19, 2017; 9(12);3072-3085 [Pubmed: 30053369]
Throughput
- Low Throughput
Curated By
- BioGRID