BAIT
AKT3
Pkbg
v-akt murine thymoma viral oncogene homolog 3
GO Process (5)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
ITPR1
I145TR, IP3R1, InsP3R, InsP3R1, P400
inositol 1,4,5-trisphosphate receptor, type 1
GO Process (15)
GO Function (8)
GO Component (24)
Gene Ontology Biological Process
- calcium ion transmembrane transport [ISO]
- calcium ion transport [ISO]
- cellular response to cAMP [IDA]
- cellular response to hypoxia [IEP]
- endoplasmic reticulum calcium ion homeostasis [ISO]
- inositol phosphate-mediated signaling [IDA, ISO, ISS]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [ISO, ISS]
- negative regulation of calcium-mediated signaling [ISO]
- negative regulation of neuron death [IMP]
- positive regulation of calcium ion transport [IDA]
- positive regulation of cytosolic calcium ion concentration [IMP]
- post-embryonic development [ISO]
- release of sequestered calcium ion into cytosol [IMP, ISO]
- response to hypoxia [ISO]
- voluntary musculoskeletal movement [ISO]
Gene Ontology Molecular Function- calcium channel inhibitor activity [ISO]
- inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity [IDA, ISO, ISS]
- intracellular ligand-gated calcium channel activity [ISO, ISS]
- phosphatidylinositol binding [ISO, ISS]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA]
- protein phosphatase binding [IDA]
- calcium channel inhibitor activity [ISO]
- inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity [IDA, ISO, ISS]
- intracellular ligand-gated calcium channel activity [ISO, ISS]
- phosphatidylinositol binding [ISO, ISS]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA]
- protein phosphatase binding [IDA]
Gene Ontology Cellular Component
- calcineurin complex [ISO]
- cell [ISO]
- cytoplasm [IDA, ISO]
- cytosol [ISO]
- dendrite [IDA]
- endoplasmic reticulum [ISO]
- endoplasmic reticulum membrane [IDA, ISO, TAS]
- intracellular [ISO]
- intracellular membrane-bounded organelle [IDA]
- membrane [ISO]
- membrane raft [IDA]
- neuronal cell body [IDA]
- nuclear envelope [IDA, ISO]
- nuclear inner membrane [ISO]
- nucleolus [ISO]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA]
- platelet dense granule membrane [ISO]
- platelet dense tubular network [ISO]
- postsynaptic density [IDA, ISO]
- protein complex [IDA, ISO]
- sarcoplasmic reticulum [IDA, ISO]
- secretory granule membrane [IDA]
- synaptic membrane [IDA]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Structural Analysis of Hippocampal Kinase Signal Transduction.
Kinases are a major clinical target for human diseases. Identifying the proteins that interact with kinases in vivo will provide information on unreported substrates and will potentially lead to more specific methods for therapeutic kinase regulation. Here, endogenous immunoprecipitations of evolutionally distinct kinases (i.e., Akt, ERK2, and CAMK2) from rodent hippocampi were analyzed by mass spectrometry to generate three highly ... [more]
ACS Chem Neurosci Dec. 19, 2017; 9(12);3072-3085 [Pubmed: 30053369]
Throughput
- Low Throughput
Curated By
- BioGRID