PREY

PSMC3

proteasome (prosome, macropain) 26S subunit, ATPase 3
GO Process (1)
GO Function (1)
GO Component (7)
Rattus norvegicus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Structural Analysis of Hippocampal Kinase Signal Transduction.

McClatchy DB, Yu NK, Martinez-Bartolome S, Patel R, Pelletier AR, Lavalle-Adam M, Powell SB, Roberto M, Yates JR

Kinases are a major clinical target for human diseases. Identifying the proteins that interact with kinases in vivo will provide information on unreported substrates and will potentially lead to more specific methods for therapeutic kinase regulation. Here, endogenous immunoprecipitations of evolutionally distinct kinases (i.e., Akt, ERK2, and CAMK2) from rodent hippocampi were analyzed by mass spectrometry to generate three highly ... [more]

ACS Chem Neurosci Dec. 19, 2017; 9(12);3072-3085 [Pubmed: 30053369]

Throughput

  • Low Throughput

Curated By

  • BioGRID