BAIT
MAPK1
Erk2
mitogen activated protein kinase 1
GO Process (37)
GO Function (12)
GO Component (19)
Gene Ontology Biological Process
- B cell receptor signaling pathway [ISO]
- ERBB signaling pathway [ISO]
- ERK1 and ERK2 cascade [ISO]
- MAPK cascade [IMP, ISO]
- MAPK import into nucleus [IDA]
- T cell receptor signaling pathway [ISO]
- caveolin-mediated endocytosis [TAS]
- cellular response to DNA damage stimulus [ISO]
- cellular response to granulocyte macrophage colony-stimulating factor stimulus [ISO]
- cellular response to organic substance [IDA]
- cytosine metabolic process [ISO]
- intracellular signal transduction [IDA, TAS]
- labyrinthine layer blood vessel development [ISO]
- lipopolysaccharide-mediated signaling pathway [ISO]
- mammary gland epithelial cell proliferation [ISO]
- negative regulation of cell differentiation [ISO]
- organ morphogenesis [ISO]
- peptidyl-serine phosphorylation [IDA, IMP, ISO]
- peptidyl-threonine phosphorylation [ISO, ISS]
- positive regulation of cell migration [IEP]
- positive regulation of cell proliferation [IEP]
- positive regulation of peptidyl-threonine phosphorylation [ISO]
- positive regulation of transcription, DNA-templated [IEP]
- positive regulation of translation [IMP]
- protein phosphorylation [IDA, ISO, TAS]
- regulation of Golgi inheritance [TAS]
- regulation of cytoskeleton organization [TAS]
- regulation of early endosome to late endosome transport [TAS]
- regulation of protein stability [ISS]
- regulation of stress-activated MAPK cascade [TAS]
- response to epidermal growth factor [ISO, ISS]
- response to estrogen [IDA]
- response to exogenous dsRNA [ISO]
- response to lipopolysaccharide [ISO]
- response to toxic substance [IDA]
- sensory perception of pain [IMP]
- signal transduction [IDA]
Gene Ontology Molecular Function- ATP binding [IDA]
- MAP kinase activity [IDA, IMP, ISO, TAS]
- RNA polymerase II carboxy-terminal domain kinase activity [ISO]
- kinase activity [ISO, TAS]
- mitogen-activated protein kinase kinase kinase binding [IPI]
- phosphatase binding [ISO]
- phosphotyrosine binding [ISO]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein kinase binding [IPI]
- protein serine/threonine kinase activity [IC, IDA, ISO, TAS]
- transcription factor binding [IPI]
- ATP binding [IDA]
- MAP kinase activity [IDA, IMP, ISO, TAS]
- RNA polymerase II carboxy-terminal domain kinase activity [ISO]
- kinase activity [ISO, TAS]
- mitogen-activated protein kinase kinase kinase binding [IPI]
- phosphatase binding [ISO]
- phosphotyrosine binding [ISO]
- protein binding [IPI]
- protein kinase activity [ISO]
- protein kinase binding [IPI]
- protein serine/threonine kinase activity [IC, IDA, ISO, TAS]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
- Golgi apparatus [TAS]
- axon [IDA]
- caveola [TAS]
- cytoplasm [ISO, ISS]
- cytoskeleton [TAS]
- cytosol [IDA, ISO, TAS]
- dendrite cytoplasm [IDA]
- early endosome [TAS]
- extracellular vesicular exosome [ISO]
- focal adhesion [TAS]
- late endosome [TAS]
- microtubule cytoskeleton [ISO]
- mitochondrion [ISO, TAS]
- mitotic spindle [ISS]
- nucleoplasm [IDA, ISO, TAS]
- nucleus [IDA, ISO, TAS]
- perikaryon [IDA]
- protein complex [IDA]
- pseudopodium [ISO]
Rattus norvegicus
PREY
RAPGEF2
PDZ-GEF1, RA-GEF-1, nRap GEP
Rap guanine nucleotide exchange factor (GEF) 2
GO Process (32)
GO Function (10)
GO Component (11)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISO, ISS]
- Rap protein signal transduction [ISO, ISS]
- adenylate cyclase-activating adrenergic receptor signaling pathway [ISO, ISS]
- blood vessel development [ISO, ISS]
- brain-derived neurotrophic factor receptor signaling pathway [IMP]
- cAMP-mediated signaling [ISO, ISS]
- cellular response to cAMP [ISO, ISS]
- cellular response to cGMP [ISO, ISS]
- cellular response to nerve growth factor stimulus [IDA]
- establishment of endothelial barrier [ISO, ISS]
- forebrain neuron development [ISO, ISS]
- negative regulation of cell proliferation [ISO, ISS]
- negative regulation of dendrite morphogenesis [ISO, ISS]
- negative regulation of melanin biosynthetic process [ISO, ISS]
- nerve growth factor signaling pathway [IDA]
- neuron migration [ISO, ISS]
- neuron projection development [ISO, ISS]
- neuropeptide signaling pathway [ISO, ISS]
- positive regulation of ERK1 and ERK2 cascade [IMP, ISO, ISS]
- positive regulation of Rap GTPase activity [IDA, IMP, ISO]
- positive regulation of Ras GTPase activity [ISO, ISS]
- positive regulation of cAMP-dependent protein kinase activity [ISO, ISS]
- positive regulation of cAMP-mediated signaling [ISO, ISS]
- positive regulation of dendritic cell apoptotic process [ISO, ISS]
- positive regulation of neuron migration [ISO, ISS]
- positive regulation of neuron projection development [IMP]
- positive regulation of protein binding [ISO, ISS]
- positive regulation of protein kinase activity [IMP, ISO, ISS]
- positive regulation of vasculogenesis [ISO, ISS]
- regulation of cell junction assembly [ISO, ISS]
- regulation of synaptic plasticity [IDA]
- ventricular system development [ISO, ISS]
Gene Ontology Molecular Function- PDZ domain binding [ISO, ISS]
- Rap GTPase activator activity [ISO]
- Rap guanyl-nucleotide exchange factor activity [IMP, ISO, ISS]
- Ras guanyl-nucleotide exchange factor activity [ISO, ISS]
- WW domain binding [ISO, ISS]
- beta-1 adrenergic receptor binding [ISO, ISS]
- cAMP binding [ISO, ISS]
- cGMP binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
- PDZ domain binding [ISO, ISS]
- Rap GTPase activator activity [ISO]
- Rap guanyl-nucleotide exchange factor activity [IMP, ISO, ISS]
- Ras guanyl-nucleotide exchange factor activity [ISO, ISS]
- WW domain binding [ISO, ISS]
- beta-1 adrenergic receptor binding [ISO, ISS]
- cAMP binding [ISO, ISS]
- cGMP binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Structural Analysis of Hippocampal Kinase Signal Transduction.
Kinases are a major clinical target for human diseases. Identifying the proteins that interact with kinases in vivo will provide information on unreported substrates and will potentially lead to more specific methods for therapeutic kinase regulation. Here, endogenous immunoprecipitations of evolutionally distinct kinases (i.e., Akt, ERK2, and CAMK2) from rodent hippocampi were analyzed by mass spectrometry to generate three highly ... [more]
ACS Chem Neurosci Dec. 19, 2017; 9(12);3072-3085 [Pubmed: 30053369]
Throughput
- Low Throughput
Curated By
- BioGRID