Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

The aftiphilin/p200/gamma-synergin complex.

Hirst J, Borner GH, Harbour M, Robinson MS

Aftiphilin is a protein that was recently identified in database searches for proteins with motifs that interact with AP-1 and clathrin, but its function is unknown. Here we demonstrate that aftiphilin has a second, atypical clathrin binding site, YQW, that colocalizes with AP-1 by immunofluorescence, and that is enriched in clathrin-coated vesicles (CCVs), confirming that it is a bona fide ... [more]

Mol Biol Cell May. 01, 2005; 16(5);2554-65 [Pubmed: 15758025]

Throughput

  • Low Throughput

Curated By

  • BioGRID