BAIT
NUPR1
COM1, P8
nuclear protein, transcriptional regulator, 1
GO Process (1)
GO Function (0)
GO Component (2)
Homo sapiens
PREY
DLG1
DLGH1, SAP-97, SAP97, dJ1061C18.1.1, hdlg
discs, large homolog 1 (Drosophila)
GO Process (17)
GO Function (11)
GO Component (17)
Gene Ontology Biological Process
- actin filament organization [IDA]
- axon guidance [TAS]
- cortical actin cytoskeleton organization [IDA]
- dephosphorylation [TAS]
- endothelial cell proliferation [IDA]
- establishment or maintenance of cell polarity [TAS]
- mitotic cell cycle checkpoint [NAS]
- negative regulation of mitotic cell cycle [IMP]
- nucleotide phosphorylation [TAS]
- positive regulation of establishment of protein localization to plasma membrane [IDA]
- positive regulation of potassium ion transport [IDA]
- protein localization to plasma membrane [IMP, TAS]
- regulation of membrane potential [IDA]
- regulation of sodium ion transmembrane transport [TAS]
- single organismal cell-cell adhesion [IDA]
- synaptic transmission [TAS]
- tight junction assembly [IDA]
Gene Ontology Molecular Function- L27 domain binding [IPI]
- cytoskeletal protein binding [TAS]
- guanylate kinase activity [TAS]
- ion channel binding [IPI]
- mitogen-activated protein kinase kinase binding [IPI]
- phosphatase binding [IPI]
- phosphoprotein phosphatase activity [TAS]
- potassium channel regulator activity [IDA, NAS]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- L27 domain binding [IPI]
- cytoskeletal protein binding [TAS]
- guanylate kinase activity [TAS]
- ion channel binding [IPI]
- mitogen-activated protein kinase kinase binding [IPI]
- phosphatase binding [IPI]
- phosphoprotein phosphatase activity [TAS]
- potassium channel regulator activity [IDA, NAS]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- MPP7-DLG1-LIN7 complex [IDA]
- basolateral plasma membrane [IDA]
- cell junction [IDA]
- cell-cell junction [IDA]
- cytoplasm [IDA]
- cytoplasmic side of plasma membrane [IDA]
- cytosol [TAS]
- endoplasmic reticulum [IDA]
- extracellular vesicular exosome [IDA]
- immunological synapse [TAS]
- intercalated disc [TAS]
- microtubule [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [TAS]
- tight junction [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
ZZW-115-dependent inhibition of NUPR1 nuclear translocation sensitizes cancer cells to genotoxic agents.
Establishing the interactome of the cancer-associated stress protein Nuclear Protein 1 (NUPR1), we found that it binds to several hundreds of proteins, including proteins involved in nuclear translocation, DNA repair, and key factors of the SUMO pathway. We demonstrated that the NUPR1 inhibitor ZZW-115, an organic synthetic molecule, competes with importins for the binding to the NLS region of NUPR1, ... [more]
JCI Insight Sep. 17, 2020; 5(18); [Pubmed: 32780723]
Throughput
- High Throughput
Curated By
- BioGRID