BAIT

PUB1

RNP1, L000001529, YNL016W
Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress
GO Process (3)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

SMX3

Sm F, SmF, L000002786, YPR182W
Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F
GO Process (1)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

RNA-binding and prion domains: the Yin and Yang of phase separation.

Gotor NL, Armaos A, Calloni G, Torrent Burgas M, Vabulas RM, De Groot NS, Tartaglia GG

Proteins and RNAs assemble in membrane-less organelles that organize intracellular spaces and regulate biochemical reactions. The ability of proteins and RNAs to form condensates is encoded in their sequences, yet it is unknown which domains drive the phase separation (PS) process and what are their specific roles. Here, we systematically investigated the human and yeast proteomes to find regions promoting ... [more]

Nucleic Acids Res Dec. 25, 2019; 48(17);9491-9504 [Pubmed: 32857852]

Throughput

  • High Throughput

Additional Notes

  • High confidence interactors were identified using a two-sample t-test statistical analysis with an FDR < 0.001.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SMX3 PUB1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.3359BioGRID
2025683

Curated By

  • BioGRID