BAIT

PTP4A3

PRL-3, PRL-R, PRL3
protein tyrosine phosphatase type IVA, member 3
GO Process (2)
GO Function (1)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens
PREY

FAM71F1

FAM137A, NYD-SP18
family with sequence similarity 71, member F1
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens

Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

The Oncogenic PRL Protein Causes Acid Addiction of Cells by Stimulating Lysosomal Exocytosis.

Funato Y, Yoshida A, Hirata Y, Hashizume O, Yamazaki D, Miki H

Extracellular pH is usually maintained around 7.4 in multicellular organisms, and cells are optimized to proliferate under this condition. Here, we find cells can adapt to a more acidic pH of 6.5 and become addicted to this acidic microenvironment by expressing phosphatase of regenerating liver (PRL), a driver of cancer malignancy. Genome-scale CRISPR-Cas9 knockout screening and subsequent analyses revealed that ... [more]

Dev Cell Nov. 23, 2020; 55(4);387-397.e8 [Pubmed: 32918875]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: growth abnormality (HP:0001507)
  • phenotype: viability (PATO:0000169)

Additional Notes

  • CRISPR GI screen
  • Cell Line: HEK293
  • Experimental Setup: Exposure to alkaline stress
  • GIST: A-phenotypic positive genetic interaction
  • Library: Geckov2
  • Significance Threshold: [FDR] < 0.05

Curated By

  • BioGRID