Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

The Oncogenic PRL Protein Causes Acid Addiction of Cells by Stimulating Lysosomal Exocytosis.

Funato Y, Yoshida A, Hirata Y, Hashizume O, Yamazaki D, Miki H

Extracellular pH is usually maintained around 7.4 in multicellular organisms, and cells are optimized to proliferate under this condition. Here, we find cells can adapt to a more acidic pH of 6.5 and become addicted to this acidic microenvironment by expressing phosphatase of regenerating liver (PRL), a driver of cancer malignancy. Genome-scale CRISPR-Cas9 knockout screening and subsequent analyses revealed that ... [more]

Dev Cell Nov. 23, 2020; 55(4);387-397.e8 [Pubmed: 32918875]

Throughput

Low Throughput

Ontology Terms

phenotype: growth abnormality (HP:0001507)
phenotype: viability (PATO:0000169)

Additional Notes

CRISPR GI screen
Cell Line: HEK293
Experimental Setup: Exposure to alkaline stress
GIST: A-phenotypic positive genetic interaction
Library: Geckov2
Significance Threshold: [FDR] < 0.05

Curated By

BioGRID

Interaction Summary

PTP4A3

Gene Ontology Biological Process

Gene Ontology Molecular Function

prenylated protein tyrosine phosphatase activity [TAS]

HIST1H3J