BAIT
PTP4A3
PRL-3, PRL-R, PRL3
protein tyrosine phosphatase type IVA, member 3
GO Process (2)
GO Function (1)
GO Component (0)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
CXCL12
IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1
chemokine (C-X-C motif) ligand 12
GO Process (17)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [TAS]
- blood circulation [TAS]
- cell adhesion [TAS]
- cell chemotaxis [IDA]
- cellular calcium ion homeostasis [TAS]
- chemokine-mediated signaling pathway [IDA]
- chemotaxis [TAS]
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage [IDA]
- negative regulation of leukocyte apoptotic process [IDA]
- negative regulation of leukocyte tethering or rolling [IDA]
- positive regulation of T cell migration [IDA]
- positive regulation of calcium ion import [TAS]
- positive regulation of cell adhesion [IDA]
- positive regulation of monocyte chemotaxis [IDA]
- regulation of actin polymerization or depolymerization [TAS]
- response to virus [TAS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
The Oncogenic PRL Protein Causes Acid Addiction of Cells by Stimulating Lysosomal Exocytosis.
Extracellular pH is usually maintained around 7.4 in multicellular organisms, and cells are optimized to proliferate under this condition. Here, we find cells can adapt to a more acidic pH of 6.5 and become addicted to this acidic microenvironment by expressing phosphatase of regenerating liver (PRL), a driver of cancer malignancy. Genome-scale CRISPR-Cas9 knockout screening and subsequent analyses revealed that ... [more]
Dev Cell Nov. 23, 2020; 55(4);387-397.e8 [Pubmed: 32918875]
Throughput
- Low Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- phenotype: viability (PATO:0000169)
Additional Notes
- CRISPR GI screen
- Cell Line: HEK293
- Experimental Setup: Exposure to alkaline stress
- GIST: A-phenotypic positive genetic interaction
- Library: Geckov2
- Significance Threshold: [FDR] < 0.05
Curated By
- BioGRID