BAIT
CEBPA
DBPCEP
CCAAT/enhancer binding protein (C/EBP), alpha
GO Process (34)
GO Function (16)
GO Component (6)
Gene Ontology Biological Process
- acute-phase response [IEP]
- brown fat cell differentiation [ISO]
- cell differentiation [IEP]
- cell maturation [ISO]
- cellular response to lithium ion [ISO]
- cellular response to organic cyclic compound [ISO]
- cholesterol metabolic process [ISO]
- embryonic placenta development [ISO]
- fat cell differentiation [ISO]
- inner ear development [ISO]
- liver development [IEP, ISO]
- lung development [ISO]
- macrophage differentiation [ISO]
- mitochondrion organization [ISO]
- myeloid cell differentiation [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [IDA]
- organ regeneration [IEP]
- positive regulation of fat cell differentiation [IMP, ISO]
- positive regulation of osteoblast differentiation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- positive regulation of transcription from RNA polymerase III promoter [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- regulation of cell proliferation [ISO]
- regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of transcription, DNA-templated [IDA, ISO, TAS]
- response to glucocorticoid [IEP]
- response to nutrient [IEP]
- response to vitamin B2 [IEP]
- transcription from RNA polymerase II promoter [ISO]
- transcription, DNA-templated [ISO]
- urea cycle [ISO]
- white fat cell differentiation [ISO]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- HMG box domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA]
- protein domain specific binding [IPI]
- protein heterodimerization activity [IDA, IPI]
- protein homodimerization activity [IDA, IPI, ISO]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
- DNA binding [IDA, ISO]
- HMG box domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA]
- protein domain specific binding [IPI]
- protein heterodimerization activity [IDA, IPI]
- protein homodimerization activity [IDA, IPI, ISO]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
PTPN11
2700084A17Rik, AW536184, PTP1D, PTP2C, SAP-2, SH-PTP2, SH-PTP3, SHP-2, Shp2, Syp
protein tyrosine phosphatase, non-receptor type 11
GO Process (41)
GO Function (12)
GO Component (8)
Gene Ontology Biological Process
- DNA damage checkpoint [IMP]
- ERBB signaling pathway [ISO]
- abortive mitotic cell cycle [IMP]
- activation of MAPK activity [IMP]
- atrioventricular canal development [ISO]
- axonogenesis [IMP]
- brain development [ISO]
- dephosphorylation [IMP]
- ephrin receptor signaling pathway [ISO]
- epidermal growth factor receptor signaling pathway [IGI]
- face morphogenesis [ISO]
- genitalia development [ISO]
- glucose homeostasis [IMP]
- heart development [ISO]
- hormone metabolic process [IMP]
- hormone-mediated signaling pathway [IDA]
- inner ear development [ISO]
- integrin-mediated signaling pathway [IMP]
- lipid metabolic process [IMP]
- megakaryocyte development [IMP]
- multicellular organismal reproductive process [IMP]
- negative regulation of cell adhesion mediated by integrin [IMP]
- negative regulation of cortisol secretion [IMP]
- negative regulation of growth hormone secretion [IMP]
- negative regulation of hormone secretion [IMP]
- negative regulation of insulin secretion [IMP]
- neurotrophin TRK receptor signaling pathway [IMP]
- organ growth [IMP]
- peptidyl-tyrosine dephosphorylation [IDA, ISO]
- platelet formation [IGI]
- platelet-derived growth factor receptor signaling pathway [IGI]
- positive regulation of ERK1 and ERK2 cascade [IGI]
- positive regulation of glucose import in response to insulin stimulus [ISO]
- positive regulation of hormone secretion [IMP]
- positive regulation of mitotic cell cycle [IGI]
- positive regulation of signal transduction [IMP, ISO]
- protein dephosphorylation [ISO]
- regulation of cell adhesion mediated by integrin [ISO]
- regulation of multicellular organism growth [IMP]
- regulation of protein export from nucleus [IMP]
- triglyceride metabolic process [IMP]
Gene Ontology Molecular Function- D1 dopamine receptor binding [ISO]
- SH3/SH2 adaptor activity [ISO]
- insulin receptor binding [ISO]
- insulin receptor substrate binding [ISO]
- non-membrane spanning protein tyrosine phosphatase activity [ISO]
- peptide hormone receptor binding [IPI]
- phospholipase binding [ISO]
- phosphoprotein phosphatase activity [IMP, ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein tyrosine phosphatase activity [IDA, ISO]
- receptor tyrosine kinase binding [IPI]
- D1 dopamine receptor binding [ISO]
- SH3/SH2 adaptor activity [ISO]
- insulin receptor binding [ISO]
- insulin receptor substrate binding [ISO]
- non-membrane spanning protein tyrosine phosphatase activity [ISO]
- peptide hormone receptor binding [IPI]
- phospholipase binding [ISO]
- phosphoprotein phosphatase activity [IMP, ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein tyrosine phosphatase activity [IDA, ISO]
- receptor tyrosine kinase binding [IPI]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Affinity purification strategies for proteomic analysis of transcription factor complexes.
Affinity purification (AP) coupled to mass spectrometry (MS) has been successful in elucidating protein molecular networks of mammalian cells. These approaches have dramatically increased the knowledge of the interconnectivity present among proteins and highlighted biological functions within different protein complexes. Despite significant technical improvements reached in the past years, it is still challenging to identify the interaction networks and the ... [more]
J Proteome Res Sep. 06, 2013; 12(9);4018-27 [Pubmed: 23937658]
Throughput
- High Throughput
Curated By
- BioGRID