BAIT

CEBPA

DBPCEP
CCAAT/enhancer binding protein (C/EBP), alpha
GO Process (34)
GO Function (16)
GO Component (6)
Rattus norvegicus
PREY

UBC

2700054O04Rik, AI194771, Rps27a, TI-225, Uba52, Ubb
ubiquitin C
GO Process (1)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Affinity purification strategies for proteomic analysis of transcription factor complexes.

Giambruno R, Grebien F, Stukalov A, Knoll C, Planyavsky M, Rudashevskaya EL, Colinge J, Superti-Furga G, Bennett KL

Affinity purification (AP) coupled to mass spectrometry (MS) has been successful in elucidating protein molecular networks of mammalian cells. These approaches have dramatically increased the knowledge of the interconnectivity present among proteins and highlighted biological functions within different protein complexes. Despite significant technical improvements reached in the past years, it is still challenging to identify the interaction networks and the ... [more]

J Proteome Res Sep. 06, 2013; 12(9);4018-27 [Pubmed: 23937658]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CEBPA UBC
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
2535574

Curated By

  • BioGRID