BAIT
CEBPA
DBPCEP
CCAAT/enhancer binding protein (C/EBP), alpha
GO Process (34)
GO Function (16)
GO Component (6)
Gene Ontology Biological Process
- acute-phase response [IEP]
- brown fat cell differentiation [ISO]
- cell differentiation [IEP]
- cell maturation [ISO]
- cellular response to lithium ion [ISO]
- cellular response to organic cyclic compound [ISO]
- cholesterol metabolic process [ISO]
- embryonic placenta development [ISO]
- fat cell differentiation [ISO]
- inner ear development [ISO]
- liver development [IEP, ISO]
- lung development [ISO]
- macrophage differentiation [ISO]
- mitochondrion organization [ISO]
- myeloid cell differentiation [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [IDA]
- organ regeneration [IEP]
- positive regulation of fat cell differentiation [IMP, ISO]
- positive regulation of osteoblast differentiation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- positive regulation of transcription from RNA polymerase III promoter [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- regulation of cell proliferation [ISO]
- regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of transcription, DNA-templated [IDA, ISO, TAS]
- response to glucocorticoid [IEP]
- response to nutrient [IEP]
- response to vitamin B2 [IEP]
- transcription from RNA polymerase II promoter [ISO]
- transcription, DNA-templated [ISO]
- urea cycle [ISO]
- white fat cell differentiation [ISO]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- HMG box domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA]
- protein domain specific binding [IPI]
- protein heterodimerization activity [IDA, IPI]
- protein homodimerization activity [IDA, IPI, ISO]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
- DNA binding [IDA, ISO]
- HMG box domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein complex binding [IDA]
- protein domain specific binding [IPI]
- protein heterodimerization activity [IDA, IPI]
- protein homodimerization activity [IDA, IPI, ISO]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
PRPF19
AA617263, AL024362, D19Wsu55e, NMP200, PSO4, Prp19, Snev
PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
GO Process (17)
GO Function (5)
GO Component (12)
Gene Ontology Biological Process
- cellular protein localization [ISO]
- double-strand break repair via nonhomologous end joining [ISO]
- generation of catalytic spliceosome for first transesterification step [IBA]
- inner cell mass cell proliferation [IMP]
- lipid biosynthetic process [IDA]
- mRNA splicing, via spliceosome [ISO]
- negative regulation of neuron differentiation [IDA]
- positive regulation of astrocyte differentiation [IDA]
- positive regulation of mRNA splicing, via spliceosome [IMP]
- positive regulation of neuron differentiation [ISA]
- proteasomal protein catabolic process [IMP, ISO]
- protein K63-linked ubiquitination [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [IBA]
- signal transduction involved in DNA damage checkpoint [ISO]
- spliceosomal complex assembly [ISO]
- spliceosomal tri-snRNP complex assembly [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- DNA replication factor A complex [ISO]
- Prp19 complex [IBA, ISO]
- U2-type catalytic step 1 spliceosome [IBA]
- catalytic step 2 spliceosome [IBA, ISO]
- cytoplasm [IDA, ISO]
- lipid particle [IDA]
- membrane [ISO]
- nuclear speck [ISO]
- nucleoplasm [ISO]
- nucleus [IDA, ISA, ISO]
- site of double-strand break [ISO]
- spliceosomal complex [IDA, ISA]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Affinity purification strategies for proteomic analysis of transcription factor complexes.
Affinity purification (AP) coupled to mass spectrometry (MS) has been successful in elucidating protein molecular networks of mammalian cells. These approaches have dramatically increased the knowledge of the interconnectivity present among proteins and highlighted biological functions within different protein complexes. Despite significant technical improvements reached in the past years, it is still challenging to identify the interaction networks and the ... [more]
J Proteome Res Sep. 06, 2013; 12(9);4018-27 [Pubmed: 23937658]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CEBPA PRPF19 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID