BAIT
C18ORF8
HsT2591, MIC1, Mic-1
chromosome 18 open reading frame 8
GO Process (0)
GO Function (0)
GO Component (1)
Gene Ontology Cellular Component
Homo sapiens
PREY
RAN
ARA24, Gsp1, TC4, OK/SW-cl.81
RAN, member RAS oncogene family
GO Process (15)
GO Function (7)
GO Component (9)
Gene Ontology Biological Process
- DNA metabolic process [TAS]
- androgen receptor signaling pathway [NAS]
- gene expression [TAS]
- intracellular transport of virus [TAS]
- mitotic nuclear division [TAS]
- mitotic spindle organization [TAS]
- positive regulation of protein binding [IDA]
- positive regulation of transcription, DNA-templated [NAS]
- protein export from nucleus [IDA]
- ribosomal large subunit export from nucleus [IMP]
- ribosomal small subunit export from nucleus [IMP]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
- viral life cycle [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export.
Cholesterol import in mammalian cells is mediated by the LDL receptor pathway. Here, we perform a genome-wide CRISPR screen using an endogenous cholesterol reporter and identify >100 genes involved in LDL-cholesterol import. We characterise C18orf8 as a core subunit of the mammalian Mon1-Ccz1 guanidine exchange factor (GEF) for Rab7, required for complex stability and function. C18orf8-deficient cells lack Rab7 activation ... [more]
Nat Commun Dec. 03, 2019; 11(1);5559 [Pubmed: 33144569]
Throughput
- High Throughput
Curated By
- BioGRID