BAIT

C18ORF8

HsT2591, MIC1, Mic-1
chromosome 18 open reading frame 8
Autophagy Project
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Homo sapiens
PREY

TUBA4A

H2-ALPHA, TUBA1
tubulin, alpha 4a
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export.

van den Boomen DJH, Sienkiewicz A, Berlin I, Jongsma MLM, van Elsland DM, Luzio JP, Neefjes JJC, Lehner PJ

Cholesterol import in mammalian cells is mediated by the LDL receptor pathway. Here, we perform a genome-wide CRISPR screen using an endogenous cholesterol reporter and identify >100 genes involved in LDL-cholesterol import. We characterise C18orf8 as a core subunit of the mammalian Mon1-Ccz1 guanidine exchange factor (GEF) for Rab7, required for complex stability and function. C18orf8-deficient cells lack Rab7 activation ... [more]

Nat Commun Dec. 03, 2019; 11(1);5559 [Pubmed: 33144569]

Throughput

  • High Throughput

Curated By

  • BioGRID