BAIT
C18ORF8
HsT2591, MIC1, Mic-1
chromosome 18 open reading frame 8
GO Process (0)
GO Function (0)
GO Component (1)
Gene Ontology Cellular Component
Homo sapiens
PREY
BAG6
BAG-6, BAT3, D6S52E, G3, DADB-70P7.10-021
BCL2-associated athanogene 6
GO Process (14)
GO Function (5)
GO Component (6)
Gene Ontology Biological Process
- brain development [ISS]
- embryo development [ISS]
- internal peptidyl-lysine acetylation [IDA]
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [ISS]
- kidney development [ISS]
- lung development [ISS]
- negative regulation of proteasomal ubiquitin-dependent protein catabolic process [ISS]
- negative regulation of proteolysis [ISS]
- protein stabilization [ISS]
- spermatogenesis [ISS]
- synaptonemal complex assembly [ISS]
- tail-anchored membrane protein insertion into ER membrane [IDA]
- ubiquitin-dependent protein catabolic process [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export.
Cholesterol import in mammalian cells is mediated by the LDL receptor pathway. Here, we perform a genome-wide CRISPR screen using an endogenous cholesterol reporter and identify >100 genes involved in LDL-cholesterol import. We characterise C18orf8 as a core subunit of the mammalian Mon1-Ccz1 guanidine exchange factor (GEF) for Rab7, required for complex stability and function. C18orf8-deficient cells lack Rab7 activation ... [more]
Nat Commun Dec. 03, 2019; 11(1);5559 [Pubmed: 33144569]
Throughput
- High Throughput
Curated By
- BioGRID