The COP9 signalosome (CSN) is an evolutionarily conserved eight-subunit (CSN1-8) protein complex that controls protein ubiquitination by deneddylating Cullin-RING E3 ligases (CRLs). The activation and function of CSN hinges on its structural dynamics, which has been challenging to decipher by conventional tools. Here, we have developed a multichemistry cross-linking mass spectrometry approach enabled by three mass spectometry-cleavable cross-linkers to generate ... [more]

Proc Natl Acad Sci U S A Dec. 25, 2019; 117(8);4088-4098 [Pubmed: 32034103]

Throughput

Low Throughput

Additional Notes

assayed using XL-MS (cross-linking coupled to mass spectrometry) using purified components

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
COPS2 COPS2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Horlbeck MA (2018)	High	-5.2339	BioGRID	2456787

Curated By

BioGRID

Interaction Summary

COPS2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]signal transducer activity [NAS]

Gene Ontology Cellular Component