Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function.

Antonin W, Holroyd C, Fasshauer D, Pabst S, Von Mollard GF, Jahn R

Sets of SNARE proteins mediate membrane fusion by assembling into core complexes. Multiple SNAREs are thought to function in different intracellular trafficking steps but it is often unclear which of the SNAREs cooperate in individual fusion reactions. We report that syntaxin 7, syntaxin 8, vti1b and endobrevin/VAMP-8 form a complex that functions in the fusion of late endosomes. Antibodies specific ... [more]

EMBO J. Dec. 01, 2000; 19(23);6453-64 [Pubmed: 11101518]

Throughput

  • Low Throughput

Curated By

  • BioGRID